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Old July 8, 2013   #3
ChrisK
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Join Date: Mar 2011
Location: Raleigh, NC
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Joseph is correct. Paper chromatography is a good way to separate plant pigments and can be done easily, it was one of the favorite lab exercises I used to teach. Making it quantitative will require some work and careful measurement. You should be able to get relative differences if not an absolute quantities. if you can get your hands on real chromatography paper that would be best. I would also recommend a dot instead of a line of extracted pigment, the smaller the better.

Depending on how fancy you want to get, old spectrometers can be found on Ebay for a couple hundred dollars...would be able to do quantitative analysis easily.

The attached doc is not mine but is a reasonable overview of the process.
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File Type: doc paperchromatography_s06.doc (59.5 KB, 15 views)
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