Forum area for discussing hybridizing tomatoes in technical terms and information pertinent to trait/variety specific long-term (1+ years) growout projects.
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#1 |
Tomatovillian™
Join Date: Mar 2006
Location: NY
Posts: 130
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I'm not clear on storing pollen for future use. If anyone has links to good info or white papers feel free to refer me. Most of the sites I'm finding aren't addressing my questions. I have a zillion questions. Top two are...
Can I use a home freezer to store the pollen in wax paper envelopes or plastic tubes? Is it fair for me to assume that pollen I collect towards the end of this season can be stored and used next season? |
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#2 |
Tomatovillian™
Join Date: Feb 2006
Location: 64079 (Missouri)
Posts: 252
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It's a pdf file so would need Acrobat Reader or similar to access: http://www.avrdc.org/LC/tomato/seedhybrid.pdf
Go to page #4 |
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#3 |
Tomatovillian™
Join Date: Feb 2006
Posts: 49
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Hello, Reign
I store pollen for hybridizing in polypropylene 1.5 ml microcentrifuge tubes. I fill the bottom of each tube with a small fluffy cotton ball (100% cotton, not the poly type). ![]() Photo: http://www.maysacres.com/ After collecting fresh pollen, I leave the tube with the cap open overnight in the fridge to allow the pollen to dry before freezing. Allow frozen pollen to warm to room temperature (takes 10 minutes or so) before using. After using, you can refreeze the tube of pollen. Heat and humidity reduce the viability of fresh and stored pollen. According to one study I have read about the storage life of tomato pollen, (reference: http://tgc.ifas.ufl.edu/vol1/v1p11.html )
I purchased microcentrifuge tubes and a small storage box from Earth Star Inc: http://www.earthstarproducts.shoppin...m/catalog.html Best wishes, Star |
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